Journal: Biomedical Reports
Article Title: Protective effect of scutellarin on myocardial cells treated with high glucose
doi: 10.3892/br.2025.2021
Figure Lengend Snippet: Scu alleviates H9c2 cell injury induced by HG. (A) The effect of Scu on the morphology of H9c2 cells induced by HG (magnification, x40). (B) The effect of Scu on the proliferative activity of H9c2 cells induced by HG. (C) The effect of Scu on the apoptotic rate of H9c2 cells induced by HG; the histogram indicates the proportion of apoptotic and necrotic cells in the Q2 + Q3 region of the quadrant diagram. (D) The effect of Scu on the levels of ROS in H9c2 cells induced by HG; the histogram compares the average fluorescence intensity of each group. The values displayed represent the mean ± standard error of the mean derived from data collected in three independent experiments. ## P<0.01 compared with the control group; * P<0.05 and ** P<0.01 compared with the model group. Scu, scutellarin; HG, high glucose; ROS, reactive oxygen species; ns, not significant.
Article Snippet: The Cell Counting Kit-8 (CCK-8) assay kit was obtained from Biosharp Life Sciences, the Annexin V-633 Apoptosis assay kit (cat. no. AD11) was acquired from Dojindo Laboratories, Inc. and the Total Reactive Oxygen Species (ROS) Assay Kit 520 nm (cat. no. 88-5930) was purchased from Thermo Fisher Scientific, Inc.
Techniques: Activity Assay, Fluorescence, Derivative Assay, Control